Proteomics and cytokine analyses distinguish myalgic encephalomyelitis/chronic fatigue syndrome cases from controls.

BACKGROUND: Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex, heterogenous disease characterized by unexplained persistent fatigue and other features including cognitive impairment, myalgias, post-exertional malaise, and immune system dysfunction. Cytokines are present in plasma and encapsulated in extracellular vesicles (EVs), but there have been only a few reports of EV characteristics and cargo in ME/CFS. Several small studies have previously described plasma proteins or protein pathways that are associated with ME/CFS. METHODS: We prepared extracellular vesicles (EVs) from frozen plasma samples from a cohort of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) cases and controls with prior published plasma cytokine and plasma proteomics data. The cytokine content of the plasma-derived extracellular vesicles was determined by a multiplex assay and differences between patients and controls were assessed. We then performed multi-omic statistical analyses that considered not only this new data, but extensive clinical data describing the health of the subjects. RESULTS: ME/CFS cases exhibited greater size and concentration of EVs in plasma. Assays of cytokine content in EVs revealed IL2 was significantly higher in cases. We observed numerous correlations among EV cytokines, among plasma cytokines, and among plasma proteins from mass spectrometry proteomics. Significant correlations between clinical data and protein levels suggest roles of particular proteins and pathways in the disease. For example, higher levels of the pro-inflammatory cytokines Granulocyte-Monocyte Colony-Stimulating Factor (CSF2) and Tumor Necrosis Factor (TNFα) were correlated with greater physical and fatigue symptoms in ME/CFS cases. Higher serine protease SERPINA5, which is involved in hemostasis, was correlated with higher SF-36 general health scores in ME/CFS. Machine learning classifiers were able to identify a list of 20 proteins that could discriminate between cases and controls, with XGBoost providing the best classification with 86.1% accuracy and a cross-validated AUROC value of 0.947. Random Forest distinguished cases from controls with 79.1% accuracy and an AUROC value of 0.891 using only 7 proteins. CONCLUSIONS: These findings add to the substantial number of objective differences in biomolecules that have been identified in individuals with ME/CFS. The observed correlations of proteins important in immune responses and hemostasis with clinical data further implicates a disturbance of these functions in ME/CFS.

Fashionable sportswear working as a body measurement collecting tool

Purpose>A two-dimensional (2D) body measurement system was implemented to study the application of sportswear design in measurement garment development. A total of 50 participants were recruited. The basic demographic information and sportswear preference data were collected through a survey to understand consumer preferences and acceptance of the new designs. The body measurements were collected through both the selected 2D measurement system and a commercial three-dimensional scanning booth to evaluate measurement accuracy.Design/methodology/approach>Finding the right size has been a long-existing problem for clothing consumers. Size problem is the most common reason for e-commerce returns and adds a high cost for retailers. One possible solution is to offer consumers an easy-to-use method that extracts accurate body measurements to be used for clothing size selection. The purpose of this research is to apply sportswear design elements on measurement garments to see if consumers’ interest in using the 2D measurement system can be increased without influencing the measurement accuracy.Findings>The results showed that the added design features increased consumers’ interest in using 2D body measuring technology without significantly influencing measurement accuracy.Originality/value>This research applied sportswear elements to convert a 2D measurement bodysuit to a fashionable clothing product. The solution resolved users’ privacy concerns and increased their acceptance and use of the technology. Other studies have not focused on using aesthetic features to improve the 2D measurement technology.